onions
Flippin' A, I love The Onion!
I am a science geek and used to work in the field doing research on various things like models of cellular tension, molecular fly genetics and carcinogenesis. While at MSU, I worked in the Heidemann lab and worked with various cell lines from chicken brains to human tumor cells. We looked at axonal growth via tension and cell adhesion via a micro manipulator needle set up. Anyway, I was lucky to get my name on two papers while there. Shout out to Phil, Kha and old man Heidemann (a damn good chef and fantastic boss). Check out the abstracts. I got 2nd author on one of the papers! Fun huh?
Open-dish incubator for live cell imaging with an inverted microscope.
Here we describe the design and fabrication of an inexpensive cell culture incubator for the stage of an inverted light microscope for use in live cell imaging. This device maintains the temperature of the cell culture at 37 degrees C with great stability and, after reaching equilibrium, provides focal stability of an image for 20-25 min with oil-immersion lenses. We describe two versions of the incubator: one for use with standard 60-mm plastic culture dishes, and the other version for imaging of cells on glass coverslips. Either can be made for less than $400. Most components are widely available commercially, and it requires only simple wiring and 3 h to assemble. Although the device is generally useful for live cell imaging on an inverted microscope, it is particularly suitable for work in which instruments are introduced into the culture, such as electrophysiology or micromanipulation. The design is based on the principle that control performance is limited by the lag time between detection and response. The key element of the design is a heated, temperature-controlled aluminum ring serving as a mini-incubator surrounding the culture vessel. For this reason, we call our design a "ringcubator."
Heidemann SR, Lamoureux P, Ngo K, Reynolds M, Buxbaum RE.
Department of Physiology, 2201 Biomed. Phys. Sci. Bldg., Michigan State University, East Lansing, MI 48824-3320, USA.
Biotechniques. 2003 Oct;35(4):708-14, 716.
The culture of chick forebrain neurons.
Dissociation of the forebrain of a single 8-day chick embryo produces > 10(7) neurons in nearly pure culture. Our methods allow 50-70% of these neurons to develop an axon and typical pyrimidal shape after 3-4 days in culture at low density (10(4) cells/cm2) by a stereotyped developmental sequence similar to that of rat hippocampal neurons. The culture method for chick forebrain neurons is unusually rapid, inexpensive, simple, and could be used in undergraduate laboratory exercises. The dissection and dissociation of the tissue are easy and rapid, requiring less than 30 min from cracking open the chicken egg to plating the cells. Axonal development by these neurons and growth for about a week do not require glial support. The neurons are grown on polylysine-treated culture surfaces in either CO2-dependent (Medium 199) or -independent (Liebovitz L15) media with 10% fetal bovine serum and a supplement based on the classic N2 supplement for neuronal culture.
Heidemann SR, Reynolds M, Ngo K, Lamoureux P.
Department of Physiology, Michigan State University, East Lansing, Michigan 48824, USA.
Methods Cell Biol. 2003;71:51-65.
I am a science geek and used to work in the field doing research on various things like models of cellular tension, molecular fly genetics and carcinogenesis. While at MSU, I worked in the Heidemann lab and worked with various cell lines from chicken brains to human tumor cells. We looked at axonal growth via tension and cell adhesion via a micro manipulator needle set up. Anyway, I was lucky to get my name on two papers while there. Shout out to Phil, Kha and old man Heidemann (a damn good chef and fantastic boss). Check out the abstracts. I got 2nd author on one of the papers! Fun huh?
Open-dish incubator for live cell imaging with an inverted microscope.
Here we describe the design and fabrication of an inexpensive cell culture incubator for the stage of an inverted light microscope for use in live cell imaging. This device maintains the temperature of the cell culture at 37 degrees C with great stability and, after reaching equilibrium, provides focal stability of an image for 20-25 min with oil-immersion lenses. We describe two versions of the incubator: one for use with standard 60-mm plastic culture dishes, and the other version for imaging of cells on glass coverslips. Either can be made for less than $400. Most components are widely available commercially, and it requires only simple wiring and 3 h to assemble. Although the device is generally useful for live cell imaging on an inverted microscope, it is particularly suitable for work in which instruments are introduced into the culture, such as electrophysiology or micromanipulation. The design is based on the principle that control performance is limited by the lag time between detection and response. The key element of the design is a heated, temperature-controlled aluminum ring serving as a mini-incubator surrounding the culture vessel. For this reason, we call our design a "ringcubator."
Heidemann SR, Lamoureux P, Ngo K, Reynolds M, Buxbaum RE.
Department of Physiology, 2201 Biomed. Phys. Sci. Bldg., Michigan State University, East Lansing, MI 48824-3320, USA.
Biotechniques. 2003 Oct;35(4):708-14, 716.
The culture of chick forebrain neurons.
Dissociation of the forebrain of a single 8-day chick embryo produces > 10(7) neurons in nearly pure culture. Our methods allow 50-70% of these neurons to develop an axon and typical pyrimidal shape after 3-4 days in culture at low density (10(4) cells/cm2) by a stereotyped developmental sequence similar to that of rat hippocampal neurons. The culture method for chick forebrain neurons is unusually rapid, inexpensive, simple, and could be used in undergraduate laboratory exercises. The dissection and dissociation of the tissue are easy and rapid, requiring less than 30 min from cracking open the chicken egg to plating the cells. Axonal development by these neurons and growth for about a week do not require glial support. The neurons are grown on polylysine-treated culture surfaces in either CO2-dependent (Medium 199) or -independent (Liebovitz L15) media with 10% fetal bovine serum and a supplement based on the classic N2 supplement for neuronal culture.
Heidemann SR, Reynolds M, Ngo K, Lamoureux P.
Department of Physiology, Michigan State University, East Lansing, Michigan 48824, USA.
Methods Cell Biol. 2003;71:51-65.
posted by Glenn Reyn at 1:08 PM
1 Comments:
Way to get published. Now I can make my very own cell culture incubator! I was going to spend my tax return on CDs, but not now...it's time for ringcubation!
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